Diagnostic Services

Essential 2 STI panel

The Essential 2 test kit is our sexual health diagnostic panel that can identify and differentiate between Chlamydia and Gonorrhoea infections; two different bacterial infections which are known to be the most common sexual transmitted infections (STI).  Using real-time PCR technology, the sample is qualitatively analysed to detect bacterial genomic material present within the sample using highly specific primer/probe sequences and thermal cycling, while the sample is continuously monitored by software-guided LEDs to quantify the abundance of the target genes.


Is a common STI which is caused by bacterial infection, Chlamydia trachomatis.  Many people will not show any signs or symptoms.  However, if left un-treated it can cause long term consequences including pelvic inflammatory disease and infertility.   Chlamydia trachomatis, is a gram-negative bacterium and intracellular parasite1. It starts out in a spore-like form called an elementary body and upon entering a host cell it transforms into a new form called a reticulate body. The bacteria then replicates until the cell ruptures, releasing the new elementary bodies into the environment to seek out new host cells and start the process all over again2. Chlamydia is transmitted through vaginal discharge or semen and genital contact as well as unprotected virginal, oral or anal sex.  Despite a few cases of antibiotic resistance there are no examples of Chlamydia strains showing natural or stable antibiotic resistance3.


Is the second most commonly reported bacterial STI caused by the gram-negative diplococci bacteria Neisseria Gonorrhoea4,5 .  This bacterium is transmitted during sexual contact including oral, anal and vaginal intercourse. The bacterium’s surface is coated with hair-like structures called pili which allow it to move, adhere to surfaces and exchange DNA6. It is able to evade the immune system by altering the antigens of its cellular surface (antigenic variation), as well as activating/deactivating certain genes (phase variation) 7,8.  Gonorrhea has developed resistance to nearly all varieties of antibiotics used to treat it and half of all infections each year are resistant to at least one antibiotic4.



Chlamydia trachomatis
(16S rRNA gene the cryptic plasmid multi-copy sequence)

Neisseria gonorrhoea
(16S rRNA and porA preudogene specific for N. gonorrhoeae)

Limit of detection

0.075 cp/μl, Chlamydia trachomatis
0.109 cp/µl, Neisseria gonorrhoea

Turn Around Time

Same day
*From sample arrival at the laboratory

Contents & Storage

Each Essential 2 test kit contains:

Genital swab, endocervical swab, urine or urethral swab.
95kPa specimen transport bag
Return address labelled UN3373 mailing bag
Sample collection instructions


Why should I buy an Essential 2 test rather than individual Screenings?

The Essential 2 is a cost effective and fast way to simultaneously identify and differentiate between two targets and is substantially cheaper than buying each test separately.

How long does it take for me to get my result?

We guarantee a same day turnaround from when your sample is received in our laboratory.

How do I you know that my test result is accurate?

Our laboratory is an ISO 15189 accredited clinical laboratory. We adhere to strict internal quality assurance measures and are committed to providing a high-quality service, to consistently deliver clinically valid results.

1. Elwell C, Mirrashidi K, Engel J. Chlamydia cell biology and pathogenesis. Nature Reviews Microbiology. 2016 Jun;14(6):385-400.

2. Nunes A, Gomes JP. Evolution, phylogeny, and molecular epidemiology of Chlamydia. Infection, Genetics and Evolution. 2014 Apr 1;23:49-64.

3. Sandoz KM, Rockey DD. Antibiotic resistance in Chlamydiae. Future microbiology. 2010 Sep;5(9):1427-42.

4. Centres for Disease Control and Prevention. 2021. Combating the Threat of Antibiotic-Resistant Gonorrhea. [online] Available at: <https://www.cdc.gov/std/gonorrhea/arg/carb.htm> [Accessed 22 April 2022].

5. Saini A, Eichenseer C, Meyers A, Frousiakis P. Septic gonococcal arthritis in a pediatric patient: Rare case report. International Journal of Surgery Case Reports. 2021 Mar 1;80:105701.

6. Eriksson J, Eriksson OS, Maudsdotter L, Palm O, Engman J, Sarkissian T, Aro H, Wallin M, Jonsson AB. Characterization of motility and piliation in pathogenic Neisseria. BMC microbiology. 2015 Dec;15(1):1-3.

7. Voter AF, Callaghan MM, Tippana R, Myong S, Dillard JP, Keck JL. Antigenic Variation in Neisseria gonorrhoeae Occurs Independently of RecQ-Mediated Unwinding of the pilE G Quadruplex. J Bacteriol. 2020 Jan 15;202(3):e00607-19. doi: 10.1128/JB.00607-19. PMID: 31740492; PMCID: PMC6964745.

8. Hill SA, Masters TL, Wachter J. Gonorrhea-an evolving disease of the new millennium. Microbial cell. 2016 Sep 5;3(9):371.